Expertise
Die Kernkompetenz der Gruppe liegt in der synthetischen Biochemie und der chemischen Biologie, die zur kreativen Gestaltung neuer Proteine oder Systeme eingesetzt werden. Dies umfasst Techniken aus der Molekularbiologie (verschiedene Klonierungsverfahren, Proteindesign, Produktion und Aufreinigung), Techniken aus der Zellbiologie (Säugetierzellen, Hefezellen, Bakterienzellen, Gen-Editing (zB CRISPR), konfokale Fluoreszenzmikroskopie), Techniken aus der Biophysik (Elektrophysiologie), Vesikelbildung, Fluorometrie, Circulardichroismus) und Chemie (posttranslationale Proteinmodifikation, native chemische Ligation, synthetische organische Chemie, physikalische Chemie und eine Reihe analytischer Techniken).
Assoziierter Wissenschaftler, Utrecht University
Dr. Arnold Boersma
E-Mail Adresse anzeigen
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
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Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy
H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1021/acs.jpcb.8b09829 | Journal of Physical Chemistry B | 2019 | |
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
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https://doi.org/10.1128/JB.00708-18 | Journal of Bacteriology | 2019 | |
DNA Nanotechnology Enters Cell Membranes
S. Huo, H. Li, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/advs.201900043 | Advanced Science | 2019 | |
Macromolecular crowding effects on energy transfer efficiency and donor-acceptor distance of hetero-FRET sensors using time-resolved fluorescence; ARTN 025002
J. L. Schwarz, H. J.; Leighton, R.; Miller, R. C.; Aplin, C. P.; Boersma, A. J.; Heikal, A. A.; Sheets, E. D.
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https://www.doi.org/10.1088/2050-6120/ab0242 | Methods and Applications in Fluorescence | 2019 | |
A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
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https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
Comparative Photophysical Studies of mCerulean3 and mTurquoise2.1 as FRET Donors
J. A. Beenken, E. Tetteh-Jada, C. P. Aplin, T. M. Kay, A. J. Boersma, A. A. Heikal, E. D. Sheets
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https://doi.org/10.1016/j.bpj.2019.11.423 | Biophysical Journal | 2020 | |
Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
Y. Zhou, S. Huo, M. Loznik, R. Göstl, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/anie.202010324 | Angewandte Chemie International Edition | 2020 | |
FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements
R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
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https://doi.org/10.1021/acs.jpcb.9b10498 | The Journal of Physical Chemistry B | 2020 | |
Investigating Novel Hetero-FRET Biosensors for Environmental Ionic Strength using Experimental and Theoretical Approaches
C. P. Aplin, R. C. Miller, T. M. Kay, A. Cembran, A. J. Boersma, A. A. Heikal, E. D. Sheets
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https://doi.org/10.1016/j.bpj.2019.11.2798 | Biophysical Journal | 2020 | |
Modular and Versatile Trans-Encoded Genetic Switches
A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/anie.202001372 | Angewandte Chemie International Edition | 2020 | |
Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy
T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2019.11.3322 | Biophysical Journal | 2020 |