Expertise
Die Kernkompetenz der Gruppe liegt in der synthetischen Biochemie und der chemischen Biologie, die zur kreativen Gestaltung neuer Proteine oder Systeme eingesetzt werden. Dies umfasst Techniken aus der Molekularbiologie (verschiedene Klonierungsverfahren, Proteindesign, Produktion und Aufreinigung), Techniken aus der Zellbiologie (Säugetierzellen, Hefezellen, Bakterienzellen, Gen-Editing (zB CRISPR), konfokale Fluoreszenzmikroskopie), Techniken aus der Biophysik (Elektrophysiologie), Vesikelbildung, Fluorometrie, Circulardichroismus) und Chemie (posttranslationale Proteinmodifikation, native chemische Ligation, synthetische organische Chemie, physikalische Chemie und eine Reihe analytischer Techniken).
Assoziierter Wissenschaftler, Utrecht University
Dr. Arnold Boersma
E-Mail Adresse anzeigen
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
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FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements
R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
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https://doi.org/10.1021/acs.jpcb.9b10498 | The Journal of Physical Chemistry B | 2020 | |
The more the merrier: effects of macromolecular crowding on the structure and dynamics of biological membranes
M. Löwe, M. Kalacheva, A. J. Boersma and A. Kedrov
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https://doi.org/10.1111/febs.15429 | The FEBS Journal | 2020 | |
Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
Macromolecular crowding effects on energy transfer efficiency and donor-acceptor distance of hetero-FRET sensors using time-resolved fluorescence; ARTN 025002
J. L. Schwarz, H. J.; Leighton, R.; Miller, R. C.; Aplin, C. P.; Boersma, A. J.; Heikal, A. A.; Sheets, E. D.
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https://www.doi.org/10.1088/2050-6120/ab0242 | Methods and Applications in Fluorescence | 2019 | |
Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy
H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1021/acs.jpcb.8b09829 | Journal of Physical Chemistry B | 2019 | |
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
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https://doi.org/10.1128/JB.00708-18 | Journal of Bacteriology | 2019 | |
Cell Wall Damage Increases Macromolecular Crowding Effects in the Escherichia Coli Cytoplasm
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/j.isci.2023.106367 | iScience | 2023 | |
Molecular Brightness Approach for Fret Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept
T. M. Kay, C. P. Aplin, R. Simonet, J. Beenken, R. C. Miller, C. Libal, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.3389/fmolb.2021.730394 | Frontiers in Molecular Biosciences | 2021 | |
A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
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https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
A Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1016/j.crmeth.2022.100184 | Cell Reports Methods | 2022 | |
A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1101/2021.02.25.432866 | bioRxiv | 2021 | |
Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy
T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2019.11.3322 | Biophysical Journal | 2020 |