Expertise
The core expertise of the group revolves around synthetic biochemistry and chemical biology that is applied to creative design of new proteins or systems. This includes techniques from molecular biology (various cloning techniques, protein design, production and purification), techniques from cell biology (mammalian cells, yeast cells, bacterial cells, gene editing (e.g. CRISPR), confocal fluorescence microscopy), techniques from biophysics (electrophysiology, vesicle formation, fluorometry, circular dichroism) and chemistry (protein post-translational modification, native chemical ligation, synthetic organic chemistry, physical chemistry and a range of analytical techniques).
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
---|---|---|---|---|
The more the merrier: effects of macromolecular crowding on the structure and dynamics of biological membranes
M. Löwe, M. Kalacheva, A. J. Boersma and A. Kedrov
|
https://doi.org/10.1111/febs.15429 | The FEBS Journal | 2020 | |
Supercharged Proteins and Polypeptides
C. Ma, A. Malessa, A. J. Boersma, K. Liu and A. Herrmann
|
https://doi.org/10.1002/adma.201905309 | Advanced Materials | 2020 | |
Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy
T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
|
https://doi.org/10.1016/j.bpj.2019.11.3322 | Biophysical Journal | 2020 | |
Molecular Brightness Approach for Fret Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept
T. M. Kay, C. P. Aplin, R. Simonet, J. Beenken, R. C. Miller, C. Libal, A. J. Boersma, E. D. Sheets and A. A. Heikal
|
https://doi.org/10.3389/fmolb.2021.730394 | Frontiers in Molecular Biosciences | 2021 | |
Modular and Versatile Trans-Encoded Genetic Switches
A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma and A. Herrmann
|
https://doi.org/10.1002/anie.202001372 | Angewandte Chemie International Edition | 2020 | |
Macromolecular crowding effects on energy transfer efficiency and donor-acceptor distance of hetero-FRET sensors using time-resolved fluorescence; ARTN 025002
J. L. Schwarz, H. J.; Leighton, R.; Miller, R. C.; Aplin, C. P.; Boersma, A. J.; Heikal, A. A.; Sheets, E. D.
|
https://www.doi.org/10.1088/2050-6120/ab0242 | Methods and Applications in Fluorescence | 2019 | |
Investigating Novel Hetero-FRET Biosensors for Environmental Ionic Strength using Experimental and Theoretical Approaches
C. P. Aplin, R. C. Miller, T. M. Kay, A. Cembran, A. J. Boersma, A. A. Heikal, E. D. Sheets
|
https://doi.org/10.1016/j.bpj.2019.11.2798 | Biophysical Journal | 2020 | |
High Macromolecular Crowding in Liposomes from Microfluidics
L. P. B. Guerzoni, A. V. C. de Goes, M. Kalacheva, J. Haduła, M. Mork, L. De Laporte and A. J. Boersma
|
https://doi.org/10.1002/advs.202201169 | Advanced Science | 2022 | |
FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements
R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
|
https://doi.org/10.1021/acs.jpcb.9b10498 | The Journal of Physical Chemistry B | 2020 | |
Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy
C. P. Aplin, R. C. Miller, T. M. Kay, A. A. Heikal, A. J. Boersma and E. D. Sheets
|
https://doi.org/10.1016/j.bpj.2021.01.035 | Biophysical Journal | 2021 | |
Effect of Donor Identity on FRET Sensor Sensitivity to Environmental Macromolecular Crowding
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, E. Johnson, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2020.11.966 | Biophysical Journal | 2021 | |
DNA Nanotechnology Enters Cell Membranes
S. Huo, H. Li, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/advs.201900043 | Advanced Science | 2019 |