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Research Group Boersma

Synthetic Biochemistry
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Expertise

The core expertise of the group revolves around synthetic biochemistry and chemical biology that is applied to creative design of new proteins or systems. This includes techniques from molecular biology (various cloning techniques, protein design, production and purification), techniques from cell biology (mammalian cells, yeast cells, bacterial cells, gene editing (e.g. CRISPR), confocal fluorescence microscopy), techniques from biophysics (electrophysiology, vesicle formation, fluorometry, circular dichroism) and chemistry (protein post-translational modification, native chemical ligation, synthetic organic chemistry, physical chemistry and a range of analytical techniques).

Associate scientist, Utrecht University

Prof. Dr. Arnold Boersma

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  • Science Support Team

    Fabian Schmitz

    T
    +49 241 80 20659
    Room
    38C.253
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  • Associate scientist, Utrecht University

    Prof. Dr. Arnold Boersma

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    Portraitfoto Dr. Arnold Boersma
  • PhD student

    Theodoros Pittas, M.Sc.

    T
    +49 241 80-20658
    Room
    38C.233
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    Theodoros Pittas
  • Project

    PArtCell

    Physiologically Crowded Artificial Cells for Relevant Drug Screens
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  • Project

    Protein behavior in living cells

    Model systems to understand protein behavior in living cells
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  • Project

    Physiologically Crowded Artificial Cells for Relevant Drug Screens

    Fluorescent probes shine light on the ‘dark matter’ in cells
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  • Project

    Quantification and consequences of macromolecular crowding in living cells

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  • Project

    Model systems to understand protein behavior in living cells

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  • Project

    Macromolecular crowding in living cells

    Quantification and consequences of macromolecular crowding in living cells
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  • Publication

    Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress

    B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
    Journal of Bacteriology Vol. 201 (10) (2019)
    https://doi.org/10.1128/JB.00708-18
  • Publication

    DNA Nanotechnology Enters Cell Membranes

    S. Huo, H. Li, A. J. Boersma and A. Herrmann
    Advanced Science Vol. 6 (10) (2019)
    https://doi.org/10.1002/advs.201900043
  • Publication

    Macromolecular crowding effects on energy transfer efficiency and donor-acceptor distance of hetero-FRET sensors using time-resolved fluorescence; ARTN 025002

    J. L. Schwarz, H. J.; Leighton, R.; Miller, R. C.; Aplin, C. P.; Boersma, A. J.; Heikal, A. A.; Sheets, E. D.
    Methods and Applications in Fluorescence Vol. 7 (2) (2019)
    https://www.doi.org/10.1088/2050-6120/ab0242
  • Publication

    Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy

    H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
    Journal of Physical Chemistry B Vol. 123 (2) 379-393 (2019)
    https://doi.org/10.1021/acs.jpcb.8b09829
  • Publication

    Supercharged Proteins and Polypeptides

    C. Ma, A. Malessa, A. J. Boersma, K. Liu and A. Herrmann
    Advanced Materials Vol. 32 (20) 1905309 (2020)
    https://doi.org/10.1002/adma.201905309
  • Publication

    Comparative Photophysical Studies of mCerulean3 and mTurquoise2.1 as FRET Donors

    J. A. Beenken, E. Tetteh-Jada, C. P. Aplin, T. M. Kay, A. J. Boersma, A. A. Heikal, E. D. Sheets
    Biophysical Journal Vol. 118 (3) 19903-19915 (2020)
    https://doi.org/10.1016/j.bpj.2019.11.423
  • Publication

    Investigating Novel Hetero-FRET Biosensors for Environmental Ionic Strength using Experimental and Theoretical Approaches

    C. P. Aplin, R. C. Miller, T. M. Kay, A. Cembran, A. J. Boersma, A. A. Heikal, E. D. Sheets
    Biophysical Journal Vol. 118 (3) 508a (2020)
    https://doi.org/10.1016/j.bpj.2019.11.2798
  • Publication

    Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy

    T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
    Biophysical Journal Vol. 118 (3) 615a (2020)
    https://doi.org/10.1016/j.bpj.2019.11.3322
  • Publication

    FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements

    R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
    The Journal of Physical Chemistry B Vol. 124 (17) 3447-3458 (2020)
    https://doi.org/10.1021/acs.jpcb.9b10498
  • Publication

    The more the merrier: effects of macromolecular crowding on the structure and dynamics of biological membranes

    M. Löwe, M. Kalacheva, A. J. Boersma and A. Kedrov
    The FEBS Journal Vol. 287 (23) 5039-5067 (2020)
    https://doi.org/10.1111/febs.15429
  • Publication

    Modular and Versatile Trans-Encoded Genetic Switches

    A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma and A. Herrmann
    Angewandte Chemie International Edition Vol. 59 (46) 20328-20332 (2020)
    https://doi.org/10.1002/anie.202001372
  • Publication

    Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound

    Y. Zhou, S. Huo, M. Loznik, R. Göstl, A. J. Boersma and A. Herrmann
    Angewandte Chemie International Edition Vol. 60 (3) 1493-1497 (2020)
    https://doi.org/10.1002/anie.202010324
  • Publication

    Chapter Three - Engineering crowding sensitivity into protein linkers

    T. Pittas, W. Zuo and A. J. Boersma
    Methods in Enzymology [Book] 51-81 (2020)
    https://doi.org/10.1016/bs.mie.2020.09.007
  • Publication

    Effect of Donor Identity on FRET Sensor Sensitivity to Environmental Macromolecular Crowding

    C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, E. Johnson, A. J. Boersma, E. D. Sheets and A. A. Heikal
    Biophysical Journal Vol. 120 (3) 125a (2021)
    https://doi.org/10.1016/j.bpj.2020.11.966
  • Publication

    Characterization of a New hetero-FRET Biosensor to Measure Environmental Ionic Strength

    C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, B. Liu, A. J. Boersma, A. A. Heikal and E. D. Sheets
    Biophysical Journal Vol. 120 (3) 125a (2021)
    https://doi.org/10.1016/j.bpj.2020.11.967
  • Publication

    Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound

    P. Zhao, S. Huo, J. Fan, J. Chen, F. Kiessling, A. J. Boersma, R. Göstl and A. Herrmann
    Angewandte Chemie International Edition Vol. 60 (26) 14707-14714 (2021)
    https://doi.org/10.1002/anie.202105404
  • Publication

    Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy

    C. P. Aplin, R. C. Miller, T. M. Kay, A. A. Heikal, A. J. Boersma and E. D. Sheets
    Biophysical Journal Vol. 120 (8) 1417-1430 (2021)
    https://doi.org/10.1016/j.bpj.2021.01.035
  • Publication

    A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast

    S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
    eLife Vol. 9 e54707 (2020)
    https://doi.org/10.7554/eLife.54707
  • Publication

    A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization

    Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
    bioRxiv (2021)
    https://doi.org/10.1101/2021.02.25.432866
  • Publication

    Molecular Brightness Approach for Fret Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

    T. M. Kay, C. P. Aplin, R. Simonet, J. Beenken, R. C. Miller, C. Libal, A. J. Boersma, E. D. Sheets and A. A. Heikal
    Frontiers in Molecular Biosciences Vol. 8 (878) (2021)
    https://doi.org/10.3389/fmolb.2021.730394
  • Publication

    High Macromolecular Crowding in Liposomes from Microfluidics

    L. P. B. Guerzoni, A. V. C. de Goes, M. Kalacheva, J. Haduła, M. Mork, L. De Laporte and A. J. Boersma
    Advanced Science Vol. 9 (27) 2201169 (2022)
    https://doi.org/10.1002/advs.202201169
  • Publication

    Cell Wall Damage Increases Macromolecular Crowding Effects in the Escherichia Coli Cytoplasm

    T. Pittas, W. Zuo and A. J. Boersma
    iScience Vol. 26 (4) 106367 (2023)
    https://doi.org/10.1016/j.isci.2023.106367
  • Publication

    A Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization

    Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
    Cell Reports Methods Vol. 2 (3) 100184 (2022)
    https://doi.org/10.1016/j.crmeth.2022.100184

Weitere Arbeitsgruppen

Working group

RG Chigrin

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Working group

RG De Laporte

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Working group

RG Göstl

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Working group

RG Hecht

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Working group

RG Heinen

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Working group

RG Herrmann

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Working group

RG Pich

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Working group

RG Rodriguez-Emmenegger

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Working group

RG Schwaneberg

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Working group

RG Wessling

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