Expertise
The core expertise of the group revolves around synthetic biochemistry and chemical biology that is applied to creative design of new proteins or systems. This includes techniques from molecular biology (various cloning techniques, protein design, production and purification), techniques from cell biology (mammalian cells, yeast cells, bacterial cells, gene editing (e.g. CRISPR), confocal fluorescence microscopy), techniques from biophysics (electrophysiology, vesicle formation, fluorometry, circular dichroism) and chemistry (protein post-translational modification, native chemical ligation, synthetic organic chemistry, physical chemistry and a range of analytical techniques).
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
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Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy
H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1021/acs.jpcb.8b09829 | Journal of Physical Chemistry B | 2019 | |
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
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https://doi.org/10.1128/JB.00708-18 | Journal of Bacteriology | 2019 | |
DNA Nanotechnology Enters Cell Membranes
S. Huo, H. Li, A. J. Boersma and A. Herrmann
|
https://doi.org/10.1002/advs.201900043 | Advanced Science | 2019 | |
Macromolecular crowding effects on energy transfer efficiency and donor-acceptor distance of hetero-FRET sensors using time-resolved fluorescence; ARTN 025002
J. L. Schwarz, H. J.; Leighton, R.; Miller, R. C.; Aplin, C. P.; Boersma, A. J.; Heikal, A. A.; Sheets, E. D.
|
https://www.doi.org/10.1088/2050-6120/ab0242 | Methods and Applications in Fluorescence | 2019 | |
A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
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https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
Comparative Photophysical Studies of mCerulean3 and mTurquoise2.1 as FRET Donors
J. A. Beenken, E. Tetteh-Jada, C. P. Aplin, T. M. Kay, A. J. Boersma, A. A. Heikal, E. D. Sheets
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https://doi.org/10.1016/j.bpj.2019.11.423 | Biophysical Journal | 2020 | |
Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
Y. Zhou, S. Huo, M. Loznik, R. Göstl, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/anie.202010324 | Angewandte Chemie International Edition | 2020 | |
FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements
R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
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https://doi.org/10.1021/acs.jpcb.9b10498 | The Journal of Physical Chemistry B | 2020 | |
Investigating Novel Hetero-FRET Biosensors for Environmental Ionic Strength using Experimental and Theoretical Approaches
C. P. Aplin, R. C. Miller, T. M. Kay, A. Cembran, A. J. Boersma, A. A. Heikal, E. D. Sheets
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https://doi.org/10.1016/j.bpj.2019.11.2798 | Biophysical Journal | 2020 | |
Modular and Versatile Trans-Encoded Genetic Switches
A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/anie.202001372 | Angewandte Chemie International Edition | 2020 | |
Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy
T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2019.11.3322 | Biophysical Journal | 2020 |