Expertise
The core expertise of the group revolves around synthetic biochemistry and chemical biology that is applied to creative design of new proteins or systems. This includes techniques from molecular biology (various cloning techniques, protein design, production and purification), techniques from cell biology (mammalian cells, yeast cells, bacterial cells, gene editing (e.g. CRISPR), confocal fluorescence microscopy), techniques from biophysics (electrophysiology, vesicle formation, fluorometry, circular dichroism) and chemistry (protein post-translational modification, native chemical ligation, synthetic organic chemistry, physical chemistry and a range of analytical techniques).
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
---|---|---|---|---|
FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements
R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
|
https://doi.org/10.1021/acs.jpcb.9b10498 | The Journal of Physical Chemistry B | 2020 | |
The more the merrier: effects of macromolecular crowding on the structure and dynamics of biological membranes
M. Löwe, M. Kalacheva, A. J. Boersma and A. Kedrov
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https://doi.org/10.1111/febs.15429 | The FEBS Journal | 2020 | |
Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
Macromolecular crowding effects on energy transfer efficiency and donor-acceptor distance of hetero-FRET sensors using time-resolved fluorescence; ARTN 025002
J. L. Schwarz, H. J.; Leighton, R.; Miller, R. C.; Aplin, C. P.; Boersma, A. J.; Heikal, A. A.; Sheets, E. D.
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https://www.doi.org/10.1088/2050-6120/ab0242 | Methods and Applications in Fluorescence | 2019 | |
Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy
H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1021/acs.jpcb.8b09829 | Journal of Physical Chemistry B | 2019 | |
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
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https://doi.org/10.1128/JB.00708-18 | Journal of Bacteriology | 2019 | |
Cell Wall Damage Increases Macromolecular Crowding Effects in the Escherichia Coli Cytoplasm
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/j.isci.2023.106367 | iScience | 2023 | |
Molecular Brightness Approach for Fret Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept
T. M. Kay, C. P. Aplin, R. Simonet, J. Beenken, R. C. Miller, C. Libal, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.3389/fmolb.2021.730394 | Frontiers in Molecular Biosciences | 2021 | |
A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
|
https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
A Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1016/j.crmeth.2022.100184 | Cell Reports Methods | 2022 | |
A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1101/2021.02.25.432866 | bioRxiv | 2021 | |
Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy
T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2019.11.3322 | Biophysical Journal | 2020 |