Expertise
Die Kernkompetenz der Gruppe liegt in der synthetischen Biochemie und der chemischen Biologie, die zur kreativen Gestaltung neuer Proteine oder Systeme eingesetzt werden. Dies umfasst Techniken aus der Molekularbiologie (verschiedene Klonierungsverfahren, Proteindesign, Produktion und Aufreinigung), Techniken aus der Zellbiologie (Säugetierzellen, Hefezellen, Bakterienzellen, Gen-Editing (zB CRISPR), konfokale Fluoreszenzmikroskopie), Techniken aus der Biophysik (Elektrophysiologie), Vesikelbildung, Fluorometrie, Circulardichroismus) und Chemie (posttranslationale Proteinmodifikation, native chemische Ligation, synthetische organische Chemie, physikalische Chemie und eine Reihe analytischer Techniken).
Assoziierter Wissenschaftler, Utrecht University
Dr. Arnold Boersma
E-Mail Adresse anzeigen
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
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A Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1016/j.crmeth.2022.100184 | Cell Reports Methods | 2022 | |
A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
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https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1101/2021.02.25.432866 | bioRxiv | 2021 | |
Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound
P. Zhao, S. Huo, J. Fan, J. Chen, F. Kiessling, A. J. Boersma, R. Göstl and A. Herrmann
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https://doi.org/10.1002/anie.202105404 | Angewandte Chemie International Edition | 2021 | |
Cell Wall Damage Increases Macromolecular Crowding Effects in the Escherichia Coli Cytoplasm
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/j.isci.2023.106367 | iScience | 2023 | |
Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
Characterization of a New hetero-FRET Biosensor to Measure Environmental Ionic Strength
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, B. Liu, A. J. Boersma, A. A. Heikal and E. D. Sheets
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https://doi.org/10.1016/j.bpj.2020.11.967 | Biophysical Journal | 2021 | |
Comparative Photophysical Studies of mCerulean3 and mTurquoise2.1 as FRET Donors
J. A. Beenken, E. Tetteh-Jada, C. P. Aplin, T. M. Kay, A. J. Boersma, A. A. Heikal, E. D. Sheets
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https://doi.org/10.1016/j.bpj.2019.11.423 | Biophysical Journal | 2020 | |
Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
Y. Zhou, S. Huo, M. Loznik, R. Göstl, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/anie.202010324 | Angewandte Chemie International Edition | 2020 | |
Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy
H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1021/acs.jpcb.8b09829 | Journal of Physical Chemistry B | 2019 | |
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
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https://doi.org/10.1128/JB.00708-18 | Journal of Bacteriology | 2019 | |
DNA Nanotechnology Enters Cell Membranes
S. Huo, H. Li, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/advs.201900043 | Advanced Science | 2019 |