Expertise
Die Kernkompetenz der Gruppe liegt in der synthetischen Biochemie und der chemischen Biologie, die zur kreativen Gestaltung neuer Proteine oder Systeme eingesetzt werden. Dies umfasst Techniken aus der Molekularbiologie (verschiedene Klonierungsverfahren, Proteindesign, Produktion und Aufreinigung), Techniken aus der Zellbiologie (Säugetierzellen, Hefezellen, Bakterienzellen, Gen-Editing (zB CRISPR), konfokale Fluoreszenzmikroskopie), Techniken aus der Biophysik (Elektrophysiologie), Vesikelbildung, Fluorometrie, Circulardichroismus) und Chemie (posttranslationale Proteinmodifikation, native chemische Ligation, synthetische organische Chemie, physikalische Chemie und eine Reihe analytischer Techniken).
Team
Projects
Publications
| Titel/Autoren | DOI-LINK | Magazine | Jahre | |
|---|---|---|---|---|
|
High Macromolecular Crowding in Liposomes from Microfluidics
L. P. B. Guerzoni, A. V. C. de Goes, M. Kalacheva, J. Haduła, M. Mork, L. De Laporte and A. J. Boersma
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https://doi.org/10.1002/advs.202201169 | Advanced Science | 2022 | |
|
A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
|
https://doi.org/10.1101/2021.02.25.432866 | bioRxiv | 2021 | |
|
Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound
P. Zhao, S. Huo, J. Fan, J. Chen, F. Kiessling, A. J. Boersma, R. Göstl and A. Herrmann
|
https://doi.org/10.1002/anie.202105404 | Angewandte Chemie International Edition | 2021 | |
|
Characterization of a New hetero-FRET Biosensor to Measure Environmental Ionic Strength
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, B. Liu, A. J. Boersma, A. A. Heikal and E. D. Sheets
|
https://doi.org/10.1016/j.bpj.2020.11.967 | Biophysical Journal | 2021 | |
|
Effect of Donor Identity on FRET Sensor Sensitivity to Environmental Macromolecular Crowding
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, E. Johnson, A. J. Boersma, E. D. Sheets and A. A. Heikal
|
https://doi.org/10.1016/j.bpj.2020.11.966 | Biophysical Journal | 2021 | |
|
Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy
C. P. Aplin, R. C. Miller, T. M. Kay, A. A. Heikal, A. J. Boersma and E. D. Sheets
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https://doi.org/10.1016/j.bpj.2021.01.035 | Biophysical Journal | 2021 | |
|
Molecular Brightness Approach for Fret Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept
T. M. Kay, C. P. Aplin, R. Simonet, J. Beenken, R. C. Miller, C. Libal, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.3389/fmolb.2021.730394 | Frontiers in Molecular Biosciences | 2021 | |
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A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
|
https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
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Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
|
Comparative Photophysical Studies of mCerulean3 and mTurquoise2.1 as FRET Donors
J. A. Beenken, E. Tetteh-Jada, C. P. Aplin, T. M. Kay, A. J. Boersma, A. A. Heikal, E. D. Sheets
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https://doi.org/10.1016/j.bpj.2019.11.423 | Biophysical Journal | 2020 | |
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Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
Y. Zhou, S. Huo, M. Loznik, R. Göstl, A. J. Boersma and A. Herrmann
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https://doi.org/10.1002/anie.202010324 | Angewandte Chemie International Edition | 2020 | |
|
FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements
R. Miller, C. Aplin, T. Kay, R. Leighton, C. Libal, R. Simonet, A. Cembran, A. Heikal, A. Boersma and E. Sheets
|
https://doi.org/10.1021/acs.jpcb.9b10498 | The Journal of Physical Chemistry B | 2020 |