Development of an In Vitro 3D-Model for Spinal Cord Injury to break the Astrocyte-Schwann Cell Barrier

Development of an In Vitro 3D-Model for Spinal Cord Injury to break the Astrocyte-Schwann Cell Barrier [Master Thesis]

As part of a multinational and multidisciplinary team, called “Mend the Gap”, the RG De Laporte aims to investigate the use of biomaterials for treating spinal cord injury (SCI) by injecting an anisotropic, soft gel, called Anisogel, into the site of the injury to serve as a bridge for growing nerve fibers. We will continue to design and optimize the Anisogel, bringing it closer to successful application in larger, most realistic models of SCI, with the help and support of our partners from the University of British Columbia (Vancouver, Canada). Furthermore, the project aims to collaborate with world leaders in the development and delivery of drug-based treatments, to realize an integrated approach that leverages the latest and most important research findings.

Project Description

As part of the Mend the Gap project you will study the behavior of Neurons, Schwann Cells (SC), and Astrocytes (AC) in a 3D Hydrogel with and without anisometric cues to induce alignment. One of the main challenges in Spinal Cord Injury repair lies in the inability of Neurons to pass through the cavity left after injury due to the glial scar produced by the AC. Even though SCs facilitate neuronal growth, they build an SC-AC barrier that worsens the permeability for neurons. However, literature-known 2D Models show that aligned cues can help neurons break this Barrier and facilitate further growth through the barrier (Achenbach P. et al., Nano Lett. 2023, 23, 6337−634). The project aims to build a combined 3D model to study this effect in a more realistic approach, using the Anisogel.

We are looking for a motivated and enthusiastic student to join our RG De Laporte. You should be enrolled in the master’s program in biotechnology, biology, chemistry, or a related field, with a strong interest in tissue engineering and regenerative medicine. A willingness to learn new skills, high proficiency in English (written and spoken), and an independent work manner are highly valued for this position.

This is what we offer

• Opportunity to be part of a cutting-edge research project aimed at solving an unsolved challenge in regenerative medicine.
• Enhance your practical skills in material research, cell biology, and tissue engineering through hands-on experience with state-of-the-art techniques.
• An inclusive and team-oriented work environment in an international, dynamic setting.
• The guidance and support of an experienced team

Your application should include your curriculum vitae and your transcript of records.

We look forward to receiving your application!