Expertise
The core expertise of the group revolves around synthetic biochemistry and chemical biology that is applied to creative design of new proteins or systems. This includes techniques from molecular biology (various cloning techniques, protein design, production and purification), techniques from cell biology (mammalian cells, yeast cells, bacterial cells, gene editing (e.g. CRISPR), confocal fluorescence microscopy), techniques from biophysics (electrophysiology, vesicle formation, fluorometry, circular dichroism) and chemistry (protein post-translational modification, native chemical ligation, synthetic organic chemistry, physical chemistry and a range of analytical techniques).
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
---|---|---|---|---|
A Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
|
https://doi.org/10.1016/j.crmeth.2022.100184 | Cell Reports Methods | 2022 | |
A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
S. N. Mouton, D. J. Thaller, M. M. Crane, I. L. Rempel, O. T. Terpstra, A. Steen, M. Kaeberlein, C. P. Lusk, A. J. Boersma and L. M. Veenhoff
|
https://doi.org/10.7554/eLife.54707 | eLife | 2020 | |
A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
|
https://doi.org/10.1101/2021.02.25.432866 | bioRxiv | 2021 | |
Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound
P. Zhao, S. Huo, J. Fan, J. Chen, F. Kiessling, A. J. Boersma, R. Göstl and A. Herrmann
|
https://doi.org/10.1002/anie.202105404 | Angewandte Chemie International Edition | 2021 | |
Cell Wall Damage Increases Macromolecular Crowding Effects in the Escherichia Coli Cytoplasm
T. Pittas, W. Zuo and A. J. Boersma
|
https://doi.org/10.1016/j.isci.2023.106367 | iScience | 2023 | |
Chapter Three - Engineering crowding sensitivity into protein linkers
T. Pittas, W. Zuo and A. J. Boersma
|
https://doi.org/10.1016/bs.mie.2020.09.007 | Methods in Enzymology [Book] | 2020 | |
Characterization of a New hetero-FRET Biosensor to Measure Environmental Ionic Strength
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, B. Liu, A. J. Boersma, A. A. Heikal and E. D. Sheets
|
https://doi.org/10.1016/j.bpj.2020.11.967 | Biophysical Journal | 2021 | |
Comparative Photophysical Studies of mCerulean3 and mTurquoise2.1 as FRET Donors
J. A. Beenken, E. Tetteh-Jada, C. P. Aplin, T. M. Kay, A. J. Boersma, A. A. Heikal, E. D. Sheets
|
https://doi.org/10.1016/j.bpj.2019.11.423 | Biophysical Journal | 2020 | |
Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound
Y. Zhou, S. Huo, M. Loznik, R. Göstl, A. J. Boersma and A. Herrmann
|
https://doi.org/10.1002/anie.202010324 | Angewandte Chemie International Edition | 2020 | |
Crowding Effects on Energy-Transfer Efficiencies of Hetero-FRET Probes As Measured Using Time-Resolved Fluorescence Anisotropy
H. J. Leopold, R. Leighton, J. Schwarz, A. J. Boersma, E. D. Sheets and A. A. Heikal
|
https://doi.org/10.1021/acs.jpcb.8b09829 | Journal of Physical Chemistry B | 2019 | |
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress
B. Q. Liu, Z. Hasrat, B. Poolman and A. J. Boersma
|
https://doi.org/10.1128/JB.00708-18 | Journal of Bacteriology | 2019 | |
DNA Nanotechnology Enters Cell Membranes
S. Huo, H. Li, A. J. Boersma and A. Herrmann
|
https://doi.org/10.1002/advs.201900043 | Advanced Science | 2019 |