Expertise
The core expertise of the group revolves around synthetic biochemistry and chemical biology that is applied to creative design of new proteins or systems. This includes techniques from molecular biology (various cloning techniques, protein design, production and purification), techniques from cell biology (mammalian cells, yeast cells, bacterial cells, gene editing (e.g. CRISPR), confocal fluorescence microscopy), techniques from biophysics (electrophysiology, vesicle formation, fluorometry, circular dichroism) and chemistry (protein post-translational modification, native chemical ligation, synthetic organic chemistry, physical chemistry and a range of analytical techniques).
Team
Projects
Publications
Titel/Autoren | DOI-LINK | Magazine | Jahre | |
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Cell Wall Damage Increases Macromolecular Crowding Effects in the Escherichia Coli Cytoplasm
T. Pittas, W. Zuo and A. J. Boersma
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https://doi.org/10.1016/j.isci.2023.106367 | iScience | 2023 | |
High Macromolecular Crowding in Liposomes from Microfluidics
L. P. B. Guerzoni, A. V. C. de Goes, M. Kalacheva, J. Haduła, M. Mork, L. De Laporte and A. J. Boersma
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https://doi.org/10.1002/advs.202201169 | Advanced Science | 2022 | |
A Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1016/j.crmeth.2022.100184 | Cell Reports Methods | 2022 | |
Molecular Brightness Approach for Fret Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept
T. M. Kay, C. P. Aplin, R. Simonet, J. Beenken, R. C. Miller, C. Libal, A. J. Boersma, E. D. Sheets and A. A. Heikal
|
https://doi.org/10.3389/fmolb.2021.730394 | Frontiers in Molecular Biosciences | 2021 | |
Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy
C. P. Aplin, R. C. Miller, T. M. Kay, A. A. Heikal, A. J. Boersma and E. D. Sheets
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https://doi.org/10.1016/j.bpj.2021.01.035 | Biophysical Journal | 2021 | |
Effect of Donor Identity on FRET Sensor Sensitivity to Environmental Macromolecular Crowding
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, E. Johnson, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2020.11.966 | Biophysical Journal | 2021 | |
Characterization of a New hetero-FRET Biosensor to Measure Environmental Ionic Strength
C. Nwachuku, E. Tetteh-Jada, M. Brink, S. Hoffman, B. Liu, A. J. Boersma, A. A. Heikal and E. D. Sheets
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https://doi.org/10.1016/j.bpj.2020.11.967 | Biophysical Journal | 2021 | |
Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound
P. Zhao, S. Huo, J. Fan, J. Chen, F. Kiessling, A. J. Boersma, R. Göstl and A. Herrmann
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https://doi.org/10.1002/anie.202105404 | Angewandte Chemie International Edition | 2021 | |
A Precise and General Fret-Based Method for Monitoring Structural Transitions in Protein Self-Organization
Q. Wan, S. N. Mouton, L. M. Veenhoff and A. J. Boersma
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https://doi.org/10.1101/2021.02.25.432866 | bioRxiv | 2021 | |
The more the merrier: effects of macromolecular crowding on the structure and dynamics of biological membranes
M. Löwe, M. Kalacheva, A. J. Boersma and A. Kedrov
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https://doi.org/10.1111/febs.15429 | The FEBS Journal | 2020 | |
Supercharged Proteins and Polypeptides
C. Ma, A. Malessa, A. J. Boersma, K. Liu and A. Herrmann
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https://doi.org/10.1002/adma.201905309 | Advanced Materials | 2020 | |
Single-Molecule Studies of Hetero-FRET Biosensors to Environmental Ionic Strength using Different Modalities of Fluorescence Correlation Spectroscopy
T. M. Kay, C. Libal, C. P. Aplin, A. J. Boersma, E. D. Sheets and A. A. Heikal
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https://doi.org/10.1016/j.bpj.2019.11.3322 | Biophysical Journal | 2020 |